That tops last year, when Amazon said Prime members purchased more than300 million items worldwide.
US e-commerce sales jumped as a whole thanks to Prime Day, according to data from Adobe Analytics.
Online shoppers spent $6.3 billion across sites on Wednesday, up by 6.4% compared to last year.
The company said it sold millions of Alexa-enabled devices during the sales event.
Other top-selling items were Laneige Lip Glowy Balm,Apple AirPodsand Bisselll’s Little Green Portable Deep Cleaner.